Extracellular Vesicles in Genome Editing and Nanomedicine

This detailed volume explores techniques for studying how the natural trafficking capability of extracellular vesicles (EVs) can be harnessed to deliver gene-editing cargo to the cells that need it. Beginning with chapters concerned with the surface biology of EVs, the book continues by covering the production, isolation, and characterization of EVs under research-grade and GMP-compliant conditions, the loading of CRISPR–Cas9 cargo into EVs by every route that is currently practical, as well as the therapeutic application of those loaded vesicles to pancreatic cancer, liver disease, glioblastoma, and inherited disorders. Written for the Springer Protocols Handbooks series, chapters include the kind of detailed description and implementation advice needed for success in the lab. Practical and up-to-date, Extracellular Vesicles in Genome Editing and Nanomedicine: From Laboratory Fundamentals to GMP Compliance serves as a guide for accelerating the translation of extracellular-vesicle-mediated CRISPR therapeutics from a promising research idea into a clinically meaningful reality.