Doubled Haploid Methods in Cereals

This volume presents methods on cereal breeding, double haploid(DH) production, and protocols for generating DH lines for breeding programs. Chapters guide readers through challenges in cereal breeding, plastid function, gene regulation, chloroplast development, Imperata cylindrica , hybridization of bread wheat with barley, and methodologies to examine changes in cellular organization and for localizing key molecular determinants through immunofluorescent assays within the context of ME. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Doubled Haploid Methods in Cereals aims to present up-to-date, reproducible approaches to inspire and empower researchers and breeders to develop, refine, and implement DH systems across diverse cereal species.